منابع مشابه
Nonintegrating foamy virus vectors.
Foamy viruses (FVs), or spumaviruses, are integrating retroviruses that have been developed as vectors. Here we generated nonintegrating foamy virus (NIFV) vectors by introducing point mutations into the highly conserved DD35E catalytic core motif of the foamy virus integrase sequence. NIFV vectors produced high-titer stocks, transduced dividing cells, and did not integrate. Cells infected with...
متن کاملFoamy Virus Vectors for HIV Gene Therapy
Highly active antiretroviral therapy (HAART) has vastly improved outcomes for patients infected with HIV, yet it is a lifelong regimen that is expensive and has significant side effects. Retroviral gene therapy is a promising alternative treatment for HIV/AIDS; however, inefficient gene delivery to hematopoietic stem cells (HSCs) has so far limited the efficacy of this approach. Foamy virus (FV...
متن کاملEfficient liver gene transfer with foamy virus vectors
BACKGROUND Liver gene transfer offers hope for the correction of genetic and acquired disorders. Efficient gene transfer in large animals can be obtained with hydrodynamic gene transfer (HGT), a method that can achieve sufficient levels of gene delivery. MATERIAL AND METHODS To test the relative efficiency between plasmid versus foamy virus (FV) vector-based liver gene transfer efficiency, we...
متن کاملRetargeted Foamy Virus Vectors Integrate Less Frequently Near Proto-oncogenes
Retroviral gene therapy offers immense potential to treat many genetic diseases and has already shown efficacy in clinical trials. However, retroviral vector mediated genotoxicity remains a major challenge and clinically relevant approaches to reduce integration near genes and proto-oncogenes are needed. Foamy retroviral vectors have several advantages over gammaretroviral and lentiviral vector...
متن کاملPackaging cell lines for simian foamy virus type 1 vectors.
Foamy viruses are nonpathogenic retroviruses that offer several unique opportunities for gene transfer in various cell types from different species. We have previously demonstrated the utility of simian foamy virus type 1 (SFV-1) as a vector system by transient expression assay (M. Wu et al., J. Virol. 72:3451-3454, 1998). In this report, we describe the first stable packaging cell lines for fo...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 2010
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.00394-10